Concentration Assay:Article Title: The immunoglobulin superfamily member 3 (IGSF3) promotes hepatocellular carcinoma progression through activation of the NF-κB pathway.
Article Snippet: .. Table S4 Primary antibodies with indicated concentration for WB, IHC, IF Antibody Dilution Company Cat No. WB IHC IF IGSF3 1:400 1:400 1:50 Bioss Bs-9196R PCNA 1:250 BOSTR BM0104 cyclin A 1:250 BOSTER A00700 cyclin E 1:400 BOSTER A00543-1 CDK1 1:400 BOSTER BM0027 CDK2 1:400 BOSTER PB0562 p-IKBα 1:400 Wanleibio WL03120 IKBα 1:400 Wanleibio WL02495 IKBα 1:200 Beyotime AF1282 p-NF-κB 1:400 Wanleibio WL02169 NF-κB 1:400 Wanleibio WL01273b NF-κB 1:200 Beyotime AF1234 GAPDH 1:800 BOSTER BM3876 Ki-67 1:200 BOSTER PB0065 Lamin B 1:1,000 Bioss bs-1840R β-action 1:800 Bioss bsm-33036M Table S5 Relationship between IGSF3 expression and clinicopathologic features (n=49) Features Relative IGSF3 expression P value Low (n=23) High (n=26) Age 0.2092 ≤50 4 9 >50 19 17 Sex 0.1944 Male 22 21 Female 1 5 AFP (μg/L) 0.5276 ≤400 15 20 >400 8 6 HBV 0.7471 Positive 7 6 Negative 16 20 Tumor diameter (cm) 0.0001 ≤5 19 7 >5 4 19 TNM stage 0.0002 I–II 18 6 III–IV 5 20 Vascular invasion 0.1250 Yes 17 24 No 6 2 Lymph node metastasis 0.0045 Yes 0 8 No 23 18 Significance figures are P values for Fisher’s exact test. ..
Article Title: The immunoglobulin superfamily member 3 (IGSF3) promotes hepatocellular carcinoma progression through activation of the NF-κB pathway
Article Snippet: .. Primary antibodies with indicated concentration for WB, IHC, IF ( ). table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Dilution Company Cat No. WB IHC IF IGSF3 1:400 1:400 1:50 Bioss Bs-9196R PCNA 1:250 BOSTR BM0104 cyclin A 1:250 BOSTER A00700 cyclin E 1:400 BOSTER A00543-1 CDK1 1:400 BOSTER BM0027 CDK2 1:400 BOSTER PB0562 p-IKBα 1:400 Wanleibio WL03120 IKBα 1:400 Wanleibio WL02495 IKBα 1:200 Beyotime AF1282 p-NF-κB 1:400 Wanleibio WL02169 NF-κB 1:400 Wanleibio WL01273b NF-κB 1:200 Beyotime AF1234 GAPDH 1:800 BOSTER BM3876 Ki-67 1:200 BOSTER PB0065 Lamin B 1:1,000 Bioss bs-1840R β-action 1:800 Bioss bsm-33036M Open in a separate window caption a8 Primary antibodies with indicated concentration for WB, IHC, IF .. We used the Costar 24 well insert system (Life Technologies, corning, USA) to perform cell migration assay.
Article Title: The immunoglobulin superfamily member 3 (IGSF3) promotes hepatocellular carcinoma progression through activation of the NF-κB pathway
Article Snippet: .. Primary antibodies with indicated concentration for WB, IHC, IF ( ). table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Dilution Company Cat No. WB IHC IF IGSF3 1:400 1:400 1:50 Bioss Bs-9196R PCNA 1:250 BOSTR BM0104 cyclin A 1:250 BOSTER A00700 cyclin E 1:400 BOSTER A00543-1 CDK1 1:400 BOSTER BM0027 CDK2 1:400 BOSTER PB0562 p-IKBα 1:400 Wanleibio WL03120 IKBα 1:400 Wanleibio WL02495 IKBα 1:200 Beyotime AF1282 p-NF-κB 1:400 Wanleibio WL02169 NF-κB 1:400 Wanleibio WL01273b NF-κB 1:200 Beyotime AF1234 GAPDH 1:800 BOSTER BM3876 Ki-67 1:200 BOSTER PB0065 Lamin B 1:1,000 Bioss bs-1840R β-action 1:800 Bioss bsm-33036M Open in a separate window caption a8 Primary antibodies with indicated concentration for WB, IHC, IF Cell proliferation, migration, and Matrigel invasion assay We used the Costar 24 well insert system (Life Technologies, corning, USA) to perform cell migration assay. ..
Western Blot:Article Title: The immunoglobulin superfamily member 3 (IGSF3) promotes hepatocellular carcinoma progression through activation of the NF-κB pathway.
Article Snippet: .. Table S4 Primary antibodies with indicated concentration for WB, IHC, IF Antibody Dilution Company Cat No. WB IHC IF IGSF3 1:400 1:400 1:50 Bioss Bs-9196R PCNA 1:250 BOSTR BM0104 cyclin A 1:250 BOSTER A00700 cyclin E 1:400 BOSTER A00543-1 CDK1 1:400 BOSTER BM0027 CDK2 1:400 BOSTER PB0562 p-IKBα 1:400 Wanleibio WL03120 IKBα 1:400 Wanleibio WL02495 IKBα 1:200 Beyotime AF1282 p-NF-κB 1:400 Wanleibio WL02169 NF-κB 1:400 Wanleibio WL01273b NF-κB 1:200 Beyotime AF1234 GAPDH 1:800 BOSTER BM3876 Ki-67 1:200 BOSTER PB0065 Lamin B 1:1,000 Bioss bs-1840R β-action 1:800 Bioss bsm-33036M Table S5 Relationship between IGSF3 expression and clinicopathologic features (n=49) Features Relative IGSF3 expression P value Low (n=23) High (n=26) Age 0.2092 ≤50 4 9 >50 19 17 Sex 0.1944 Male 22 21 Female 1 5 AFP (μg/L) 0.5276 ≤400 15 20 >400 8 6 HBV 0.7471 Positive 7 6 Negative 16 20 Tumor diameter (cm) 0.0001 ≤5 19 7 >5 4 19 TNM stage 0.0002 I–II 18 6 III–IV 5 20 Vascular invasion 0.1250 Yes 17 24 No 6 2 Lymph node metastasis 0.0045 Yes 0 8 No 23 18 Significance figures are P values for Fisher’s exact test. ..
Article Title: The immunoglobulin superfamily member 3 (IGSF3) promotes hepatocellular carcinoma progression through activation of the NF-κB pathway
Article Snippet: .. Primary antibodies with indicated concentration for WB, IHC, IF ( ). table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Dilution Company Cat No. WB IHC IF IGSF3 1:400 1:400 1:50 Bioss Bs-9196R PCNA 1:250 BOSTR BM0104 cyclin A 1:250 BOSTER A00700 cyclin E 1:400 BOSTER A00543-1 CDK1 1:400 BOSTER BM0027 CDK2 1:400 BOSTER PB0562 p-IKBα 1:400 Wanleibio WL03120 IKBα 1:400 Wanleibio WL02495 IKBα 1:200 Beyotime AF1282 p-NF-κB 1:400 Wanleibio WL02169 NF-κB 1:400 Wanleibio WL01273b NF-κB 1:200 Beyotime AF1234 GAPDH 1:800 BOSTER BM3876 Ki-67 1:200 BOSTER PB0065 Lamin B 1:1,000 Bioss bs-1840R β-action 1:800 Bioss bsm-33036M Open in a separate window caption a8 Primary antibodies with indicated concentration for WB, IHC, IF .. We used the Costar 24 well insert system (Life Technologies, corning, USA) to perform cell migration assay.
Article Title: The immunoglobulin superfamily member 3 (IGSF3) promotes hepatocellular carcinoma progression through activation of the NF-κB pathway
Article Snippet: .. Primary antibodies with indicated concentration for WB, IHC, IF ( ). table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Dilution Company Cat No. WB IHC IF IGSF3 1:400 1:400 1:50 Bioss Bs-9196R PCNA 1:250 BOSTR BM0104 cyclin A 1:250 BOSTER A00700 cyclin E 1:400 BOSTER A00543-1 CDK1 1:400 BOSTER BM0027 CDK2 1:400 BOSTER PB0562 p-IKBα 1:400 Wanleibio WL03120 IKBα 1:400 Wanleibio WL02495 IKBα 1:200 Beyotime AF1282 p-NF-κB 1:400 Wanleibio WL02169 NF-κB 1:400 Wanleibio WL01273b NF-κB 1:200 Beyotime AF1234 GAPDH 1:800 BOSTER BM3876 Ki-67 1:200 BOSTER PB0065 Lamin B 1:1,000 Bioss bs-1840R β-action 1:800 Bioss bsm-33036M Open in a separate window caption a8 Primary antibodies with indicated concentration for WB, IHC, IF Cell proliferation, migration, and Matrigel invasion assay We used the Costar 24 well insert system (Life Technologies, corning, USA) to perform cell migration assay. ..
Immunohistochemistry:Article Title: The immunoglobulin superfamily member 3 (IGSF3) promotes hepatocellular carcinoma progression through activation of the NF-κB pathway.
Article Snippet: .. Table S4 Primary antibodies with indicated concentration for WB, IHC, IF Antibody Dilution Company Cat No. WB IHC IF IGSF3 1:400 1:400 1:50 Bioss Bs-9196R PCNA 1:250 BOSTR BM0104 cyclin A 1:250 BOSTER A00700 cyclin E 1:400 BOSTER A00543-1 CDK1 1:400 BOSTER BM0027 CDK2 1:400 BOSTER PB0562 p-IKBα 1:400 Wanleibio WL03120 IKBα 1:400 Wanleibio WL02495 IKBα 1:200 Beyotime AF1282 p-NF-κB 1:400 Wanleibio WL02169 NF-κB 1:400 Wanleibio WL01273b NF-κB 1:200 Beyotime AF1234 GAPDH 1:800 BOSTER BM3876 Ki-67 1:200 BOSTER PB0065 Lamin B 1:1,000 Bioss bs-1840R β-action 1:800 Bioss bsm-33036M Table S5 Relationship between IGSF3 expression and clinicopathologic features (n=49) Features Relative IGSF3 expression P value Low (n=23) High (n=26) Age 0.2092 ≤50 4 9 >50 19 17 Sex 0.1944 Male 22 21 Female 1 5 AFP (μg/L) 0.5276 ≤400 15 20 >400 8 6 HBV 0.7471 Positive 7 6 Negative 16 20 Tumor diameter (cm) 0.0001 ≤5 19 7 >5 4 19 TNM stage 0.0002 I–II 18 6 III–IV 5 20 Vascular invasion 0.1250 Yes 17 24 No 6 2 Lymph node metastasis 0.0045 Yes 0 8 No 23 18 Significance figures are P values for Fisher’s exact test. ..
Article Title: The immunoglobulin superfamily member 3 (IGSF3) promotes hepatocellular carcinoma progression through activation of the NF-κB pathway
Article Snippet: .. Primary antibodies with indicated concentration for WB, IHC, IF ( ). table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Dilution Company Cat No. WB IHC IF IGSF3 1:400 1:400 1:50 Bioss Bs-9196R PCNA 1:250 BOSTR BM0104 cyclin A 1:250 BOSTER A00700 cyclin E 1:400 BOSTER A00543-1 CDK1 1:400 BOSTER BM0027 CDK2 1:400 BOSTER PB0562 p-IKBα 1:400 Wanleibio WL03120 IKBα 1:400 Wanleibio WL02495 IKBα 1:200 Beyotime AF1282 p-NF-κB 1:400 Wanleibio WL02169 NF-κB 1:400 Wanleibio WL01273b NF-κB 1:200 Beyotime AF1234 GAPDH 1:800 BOSTER BM3876 Ki-67 1:200 BOSTER PB0065 Lamin B 1:1,000 Bioss bs-1840R β-action 1:800 Bioss bsm-33036M Open in a separate window caption a8 Primary antibodies with indicated concentration for WB, IHC, IF .. We used the Costar 24 well insert system (Life Technologies, corning, USA) to perform cell migration assay.
Article Title: The immunoglobulin superfamily member 3 (IGSF3) promotes hepatocellular carcinoma progression through activation of the NF-κB pathway
Article Snippet: .. Primary antibodies with indicated concentration for WB, IHC, IF ( ). table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Dilution Company Cat No. WB IHC IF IGSF3 1:400 1:400 1:50 Bioss Bs-9196R PCNA 1:250 BOSTR BM0104 cyclin A 1:250 BOSTER A00700 cyclin E 1:400 BOSTER A00543-1 CDK1 1:400 BOSTER BM0027 CDK2 1:400 BOSTER PB0562 p-IKBα 1:400 Wanleibio WL03120 IKBα 1:400 Wanleibio WL02495 IKBα 1:200 Beyotime AF1282 p-NF-κB 1:400 Wanleibio WL02169 NF-κB 1:400 Wanleibio WL01273b NF-κB 1:200 Beyotime AF1234 GAPDH 1:800 BOSTER BM3876 Ki-67 1:200 BOSTER PB0065 Lamin B 1:1,000 Bioss bs-1840R β-action 1:800 Bioss bsm-33036M Open in a separate window caption a8 Primary antibodies with indicated concentration for WB, IHC, IF Cell proliferation, migration, and Matrigel invasion assay We used the Costar 24 well insert system (Life Technologies, corning, USA) to perform cell migration assay. ..
Expressing:Article Title: The immunoglobulin superfamily member 3 (IGSF3) promotes hepatocellular carcinoma progression through activation of the NF-κB pathway.
Article Snippet: .. Table S4 Primary antibodies with indicated concentration for WB, IHC, IF Antibody Dilution Company Cat No. WB IHC IF IGSF3 1:400 1:400 1:50 Bioss Bs-9196R PCNA 1:250 BOSTR BM0104 cyclin A 1:250 BOSTER A00700 cyclin E 1:400 BOSTER A00543-1 CDK1 1:400 BOSTER BM0027 CDK2 1:400 BOSTER PB0562 p-IKBα 1:400 Wanleibio WL03120 IKBα 1:400 Wanleibio WL02495 IKBα 1:200 Beyotime AF1282 p-NF-κB 1:400 Wanleibio WL02169 NF-κB 1:400 Wanleibio WL01273b NF-κB 1:200 Beyotime AF1234 GAPDH 1:800 BOSTER BM3876 Ki-67 1:200 BOSTER PB0065 Lamin B 1:1,000 Bioss bs-1840R β-action 1:800 Bioss bsm-33036M Table S5 Relationship between IGSF3 expression and clinicopathologic features (n=49) Features Relative IGSF3 expression P value Low (n=23) High (n=26) Age 0.2092 ≤50 4 9 >50 19 17 Sex 0.1944 Male 22 21 Female 1 5 AFP (μg/L) 0.5276 ≤400 15 20 >400 8 6 HBV 0.7471 Positive 7 6 Negative 16 20 Tumor diameter (cm) 0.0001 ≤5 19 7 >5 4 19 TNM stage 0.0002 I–II 18 6 III–IV 5 20 Vascular invasion 0.1250 Yes 17 24 No 6 2 Lymph node metastasis 0.0045 Yes 0 8 No 23 18 Significance figures are P values for Fisher’s exact test. ..
Migration:Article Title: The immunoglobulin superfamily member 3 (IGSF3) promotes hepatocellular carcinoma progression through activation of the NF-κB pathway
Article Snippet: .. Primary antibodies with indicated concentration for WB, IHC, IF ( ). table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Dilution Company Cat No. WB IHC IF IGSF3 1:400 1:400 1:50 Bioss Bs-9196R PCNA 1:250 BOSTR BM0104 cyclin A 1:250 BOSTER A00700 cyclin E 1:400 BOSTER A00543-1 CDK1 1:400 BOSTER BM0027 CDK2 1:400 BOSTER PB0562 p-IKBα 1:400 Wanleibio WL03120 IKBα 1:400 Wanleibio WL02495 IKBα 1:200 Beyotime AF1282 p-NF-κB 1:400 Wanleibio WL02169 NF-κB 1:400 Wanleibio WL01273b NF-κB 1:200 Beyotime AF1234 GAPDH 1:800 BOSTER BM3876 Ki-67 1:200 BOSTER PB0065 Lamin B 1:1,000 Bioss bs-1840R β-action 1:800 Bioss bsm-33036M Open in a separate window caption a8 Primary antibodies with indicated concentration for WB, IHC, IF Cell proliferation, migration, and Matrigel invasion assay We used the Costar 24 well insert system (Life Technologies, corning, USA) to perform cell migration assay. ..
Invasion Assay:Article Title: The immunoglobulin superfamily member 3 (IGSF3) promotes hepatocellular carcinoma progression through activation of the NF-κB pathway
Article Snippet: .. Primary antibodies with indicated concentration for WB, IHC, IF ( ). table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Dilution Company Cat No. WB IHC IF IGSF3 1:400 1:400 1:50 Bioss Bs-9196R PCNA 1:250 BOSTR BM0104 cyclin A 1:250 BOSTER A00700 cyclin E 1:400 BOSTER A00543-1 CDK1 1:400 BOSTER BM0027 CDK2 1:400 BOSTER PB0562 p-IKBα 1:400 Wanleibio WL03120 IKBα 1:400 Wanleibio WL02495 IKBα 1:200 Beyotime AF1282 p-NF-κB 1:400 Wanleibio WL02169 NF-κB 1:400 Wanleibio WL01273b NF-κB 1:200 Beyotime AF1234 GAPDH 1:800 BOSTER BM3876 Ki-67 1:200 BOSTER PB0065 Lamin B 1:1,000 Bioss bs-1840R β-action 1:800 Bioss bsm-33036M Open in a separate window caption a8 Primary antibodies with indicated concentration for WB, IHC, IF Cell proliferation, migration, and Matrigel invasion assay We used the Costar 24 well insert system (Life Technologies, corning, USA) to perform cell migration assay. ..
Cell Migration Assay:Article Title: The immunoglobulin superfamily member 3 (IGSF3) promotes hepatocellular carcinoma progression through activation of the NF-κB pathway
Article Snippet: .. Primary antibodies with indicated concentration for WB, IHC, IF ( ). table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Dilution Company Cat No. WB IHC IF IGSF3 1:400 1:400 1:50 Bioss Bs-9196R PCNA 1:250 BOSTR BM0104 cyclin A 1:250 BOSTER A00700 cyclin E 1:400 BOSTER A00543-1 CDK1 1:400 BOSTER BM0027 CDK2 1:400 BOSTER PB0562 p-IKBα 1:400 Wanleibio WL03120 IKBα 1:400 Wanleibio WL02495 IKBα 1:200 Beyotime AF1282 p-NF-κB 1:400 Wanleibio WL02169 NF-κB 1:400 Wanleibio WL01273b NF-κB 1:200 Beyotime AF1234 GAPDH 1:800 BOSTER BM3876 Ki-67 1:200 BOSTER PB0065 Lamin B 1:1,000 Bioss bs-1840R β-action 1:800 Bioss bsm-33036M Open in a separate window caption a8 Primary antibodies with indicated concentration for WB, IHC, IF Cell proliferation, migration, and Matrigel invasion assay We used the Costar 24 well insert system (Life Technologies, corning, USA) to perform cell migration assay. ..
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